Evidence record
Would evolution repeat if it ran again?
Every sentence on the exploration belongs to at least one claim below. Each says what kind of statement it is, what it rests on, what the evidence does not establish, and how far it was checked. Nothing here is a score, and none of it is a verdict on a belief or a theory.
Last checked 24 September 2026.
- Source checked: 35
- A judgement, not a fact: 12
- Partly checked: 3
- Presented as open: 1
If something here is wrong, misquoted, out of date or unfair, write to support@unknownoceans.com and give the claim number, which does not change.
What the question takes for granted
A scientific question about how far evolution repeats itself. The page answers it with one long experiment on bacteria, one review of convergence in nature and one forecasting method for influenza, and says what each can and cannot carry.
Evolution can be run again from the same starting point
Established
Not for the history of life, which cannot be replayed, but for bacteria kept frozen at intervals, which can be revived and evolved again. That is what the replay experiments did.
Repeatability and contingency are alternatives
Contested
The page argues they are not: parallel change and history-dependent change both occurred in the same experiment, and the question is how much of each.
What happens in flasks of bacteria says something about the history of life
Open
The experiment was built to test contingency under controlled conditions, which is also what limits how far it generalises. The page presents the scale-up as unanswered.
How the question is labelled
Claim: The same tape, twice
- Kind
- Framing. Signposting. It asserts nothing a source could check.
The title borrows the image of replaying the tape of life, which the page explains in its opening.
Claim: Replaying
- Kind
- Framing. Signposting. It asserts nothing a source could check.
The question
Claim: Twelve identical populations of bacteria have been running the same experiment since 1988.
- Kind
- Historical claim. A statement about the past that historical evidence can test.
- Review
- Source checked. The cited passage was read, and it says this.
Identical at the start: the populations were founded from one clone and differ only by a neutral marker that tells six from the other six.
Supports
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: Twelve initially identical populations of Escherichia coli were founded in 1988 to investigate this issue.
Supports
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: after 30 years of the Lenski experiment
Claim 3 · checked 24 September 2026 · AI-assisted audit
Claim: One of them did something the others never did.
- Kind
- Observation. Something measured or seen, as the named source reports it.
- Review
- Source checked. The cited passage was read, and it says this.
The thing it did is use citrate for growth in the presence of oxygen.
Supports
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: No population evolved the capacity to exploit citrate for >30,000 generations, although each population tested billions of mutations.
Supports
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: it still has evolved in only one of the twelve E. coli populations after 30 years of the Lenski experiment
Later work looked for
Europe PMC, 2012 on, "long-term evolution experiment" and citrate: Leon and colleagues (2018) report citrate use still in only one of the twelve populations after 30 years and find more than one potentiating step; Van Hofwegen and colleagues (2016) dispute the rarity. Both added and the page updated.
Claim 4 · checked 24 September 2026 · AI-assisted audit
Claim: Evolution repeats itself constantly at small scales, which makes it partly predictable.
- Kind
- This page’s reading. A judgement the page makes from the evidence. Others read it differently.
- Review
- A judgement, not a fact. Reviewed for whether it is fairly put and follows from the checked claims. No source can confirm it.
The page’s summary of the parallel changes in the experiment and of convergence in nature, both covered and sourced below.
Context
Historical contingency and the evolution of a key innovation in an experimental population of Escherichia coliintroduction, paragraph 4
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: Previous analyses of this experiment have shown numerous examples of parallel phenotypic and genetic evolution.
Context
Convergence, adaptation, and constraintintroduction
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Texts · Review of the field
The source reads: Convergent evolution–the independent evolution of similar features in different evolutionary lineages–is a common, perhaps ubiquitous
Claim 5 · checked 24 September 2026 · AI-assisted audit
Claim: It also depends on history in ways that can be tested experimentally
- Kind
- This page’s reading. A judgement the page makes from the evidence. Others read it differently.
- Review
- A judgement, not a fact. Reviewed for whether it is fairly put and follows from the checked claims. No source can confirm it.
Context
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: The role of historical contingency in evolution has been much debated, but rarely tested.
Corrected
24 September 2026. Previously: “It also depends on history in ways that can be demonstrated experimentally, and the experiment that demonstrates it best” What the citrate experiment demonstrates is disputed (Van Hofwegen and colleagues, 2016), and the page now reports the dispute; the summary no longer settles it.
Claim 6 · checked 24 September 2026 · AI-assisted audit
Claim: and the experiment that tests it best
- Kind
- This page’s reading. A judgement the page makes from the evidence. Others read it differently.
- Review
- A judgement, not a fact. Reviewed for whether it is fairly put and follows from the checked claims. No source can confirm it.
The page’s judgement. The authors present it as a direct test of contingency; the page reports below that others dispute what it shows.
Claim 7 · checked 24 September 2026 · AI-assisted audit
Claim: took twenty years and thirty thousand generations
- Kind
- Historical claim. A statement about the past that historical evidence can test.
- Review
- Source checked. The cited passage was read, and it says this.
The innovation appeared after about 31,500 generations; the paper that tested it by replay was published twenty years into the experiment.
Supports
Historical contingency and the evolution of a key innovation in an experimental population of Escherichia coliResults, first section; abstract
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: over the 20 years of this experiment ... finally evolved in one population by 31,500 generations
Claim 8 · checked 24 September 2026 · AI-assisted audit
Claim: The thought experiment is old: rewind life to some point in the distant past, let it run again, and see whether anything recognisable appears.
- Kind
- Historical claim. A statement about the past that historical evidence can test.
- Review
- Source checked. The cited passage was read, and it says this.
The thought experiment is Stephen Jay Gould’s replay of the tape of life.
Supports
Historical contingency and the evolution of a key innovation in an experimental population of Escherichia coliintroduction, paragraph 2
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Texts · Review of the field
The source reads: he famously suggested that replaying the ... from some point in the distant past would yield a living world far different from the one we see today
Corrected
24 September 2026. Previously: “rewind life to the beginning, let it run again” Gould’s thought experiment, as the cited paper states it, replays the tape from some point in the distant past, not from the beginning of life.
Claim 9 · checked 24 September 2026 · AI-assisted audit
Claim: It stopped being only a thought experiment when someone started twelve identical populations and kept them going for decades.
- Kind
- Historical claim. A statement about the past that historical evidence can test.
- Review
- Source checked. The cited passage was read, and it says this.
The someone is Richard Lenski, whose laboratory founded the populations.
Supports
Historical contingency and the evolution of a key innovation in an experimental population of Escherichia coliintroduction, paragraph 3
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: To address the repeatability of evolutionary trajectories and outcomes, the long-term evolution experiment (LTEE) with Escherichia coli was started in 1988 with the founding of 12 populations from the same clone
Supports
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: after 30 years of the Lenski experiment
Claim 10 · checked 24 September 2026 · AI-assisted audit
What was measured
Claim: What was measured
- Kind
- Framing. Signposting. It asserts nothing a source could check.
The experiment
Claim: The experiment
- Kind
- Framing. Signposting. It asserts nothing a source could check.
Claim: Since 1988, twelve populations of the same bacterium have been grown in the same medium, transferred daily, with samples frozen every 500 generations.
- Kind
- Historical claim. A statement about the past that historical evidence can test.
- Review
- Source checked. The cited passage was read, and it says this.
Supports
Historical contingency and the evolution of a key innovation in an experimental population of Escherichia coliintroduction, paragraph 3
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: They have since been propagated by daily 1:100 serial transfer in DM25 ... samples have been frozen every 500 generations
Supports
Sustained fitness gains and variability in fitness trajectories in the long-term evolution experiment with Escherichia coliMaterial and methods (b)
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: Every 500 generations (75 days), samples of each population are stored in 10% glycerol
Claim 13 · checked 24 September 2026 · AI-assisted audit
Claim: The frozen samples are a living fossil record that can be revived and replayed.
- Kind
- Observation. Something measured or seen, as the named source reports it.
- Review
- Source checked. The cited passage was read, and it says this.
Supports
Historical contingency and the evolution of a key innovation in an experimental population of Escherichia coliintroduction, paragraph 3
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: providing a rich ... fossil record ... Moreover, these samples remain viable, allowing us to perform simultaneous measurements and other experiments with bacteria from different generations.
Supports
Sustained fitness gains and variability in fitness trajectories in the long-term evolution experiment with Escherichia coliMaterial and methods (b)
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: the bacteria in those samples remain viable and are available for later study
Later work looked for
Europe PMC, 2012 on, "long-term evolution experiment": later work, including Leon and colleagues (2018), still revives archived clones; nothing that changes it.
Claim 14 · checked 24 September 2026 · AI-assisted audit
Claim: Much of what happened was parallel. All twelve grew larger cells, all twelve improved their growth rate, and the same genes were hit repeatedly across independent lines.
- Kind
- Observation. Something measured or seen, as the named source reports it.
- Review
- Source checked. The cited passage was read, and it says this.
Supports
Historical contingency and the evolution of a key innovation in an experimental population of Escherichia coliintroduction, paragraphs 4 and 5
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: All evolved higher maximum growth rates on glucose, shorter lag phases upon transfer into fresh medium, reduced peak population densities, and larger average cell sizes relative to their ancestor ... At least three genes have substitutions in all 12 populations
Qualifies
Historical contingency and the evolution of a key innovation in an experimental population of Escherichia coliintroduction, paragraph 5
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: while the same genes often harbor substitutions, the precise location and details of the mutations almost always differ between the populations
Supports
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: Mutations in nadR have appeared and swept to fixation in all twelve LTEE populations.
What this does not establish
Parallel at the level of genes, not of individual mutations: the exact changes almost always differ between populations.
Later work looked for
Europe PMC, 2012 on, "long-term evolution experiment": Leon and colleagues (2018) report further genes changed in all or nearly all populations; nothing that changes it.
Claim 15 · checked 24 September 2026 · AI-assisted audit
Claim: At that level evolution is repeatable and to a degree predictable.
- Kind
- This page’s reading. A judgement the page makes from the evidence. Others read it differently.
- Review
- A judgement, not a fact. Reviewed for whether it is fairly put and follows from the checked claims. No source can confirm it.
Follows from the parallel changes in the sentence before; "to a degree" is carried by the divergence the same paper reports.
Context
Historical contingency and the evolution of a key innovation in an experimental population of Escherichia coliintroduction, paragraph 5
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: At the same time, there has also been some divergence between populations.
Claim 16 · checked 24 September 2026 · AI-assisted audit
Improvement that has not stopped
Claim: Improvement that has not stopped
- Kind
- Experimental result. What an experiment or trial found, under its own conditions. A result in animals, cells or a simulation is not a result in people.
- Review
- Source checked. The cited passage was read, and it says this.
Supports
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: In all nine populations tested, the estimated mean fitness increased over both the 40 000–50 000- and the 50 000–60 000-generation intervals
What this does not establish
Through 60,000 generations, in the nine populations that could be measured.
Later work looked for
Crossref and Europe PMC, 2016 on, "long-term evolution experiment" fitness trajectory and power law: no later measurement of the fitness trajectory that changes it.
Corrected
24 September 2026. Previously: “Improvement that does not stop” The data run to 60,000 generations. A curve without a ceiling fits them better, which is not a demonstration that improvement never stops; the paper itself says "indefinitely–or at least for a long time".
Claim 17 · checked 24 September 2026 · AI-assisted audit
Claim: A 2015 analysis of the same populations addressed a simpler question: does adaptation run out.
- Kind
- Historical claim. A statement about the past that historical evidence can test.
- Review
- Source checked. The cited passage was read, and it says this.
The 2015 paper tested a prediction made by an earlier study of the first 50,000 generations.
Supports
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: it is interesting to ask whether an evolving population’s mean fitness can increase indefinitely, and potentially without any limit, even in a constant environment
Claim 18 · checked 24 September 2026 · AI-assisted audit
Claim: The expectation was that fitness would approach a ceiling as the easy improvements were used up.
- Kind
- What scholars argue. A report of what specialists argue, or where they disagree.
- Review
- Source checked. The cited passage was read, and it says this.
Supports
Sustained fitness gains and variability in fitness trajectories in the long-term evolution experiment with Escherichia coliIntroduction, paragraph 1
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Texts · Review of the field
The source reads: it is also often presumed that evolving organisms must eventually ‘run out’ of ways to improve, absent changes in their environment
Claim 19 · checked 24 September 2026 · AI-assisted audit
Claim: Not yet. In all nine populations measured, fitness was still rising between forty and sixty thousand generations, more slowly than before
- Kind
- Experimental result. What an experiment or trial found, under its own conditions. A result in animals, cells or a simulation is not a result in people.
- Review
- Source checked. The cited passage was read, and it says this.
Supports
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: In all nine populations tested, the estimated mean fitness increased over both the 40 000–50 000- and the 50 000–60 000-generation intervals ... the cumulative increase between 40 000 and 60 000 generations is highly significant
Supports
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: the rate of fitness gain declines over time
What this does not establish
Six of the nine rose significantly in both ten-thousand-generation intervals and three in only one; the rise over the whole twenty thousand is significant in all nine.
Later work looked for
Crossref and Europe PMC, 2016 on, "long-term evolution experiment" fitness trajectory and power law: nothing that changes it.
Corrected
24 September 2026. Previously: “It does not. Fitness continues to rise after fifty thousand generations, more slowly but without any sign of a limit” The paper measured nine populations to 60,000 generations. That shows fitness had not stopped rising, not that it never will, and "without any sign of a limit" stated a model comparison as an observation.
Claim 20 · checked 24 September 2026 · AI-assisted audit
Claim: and the trajectories are better described by a curve with no ceiling than by one with one.
- Kind
- What a model says. What a theory or model predicts or describes, which is not the same as what has been observed.
- Review
- Source checked. The cited passage was read, and it says this.
Supports
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Model
The source reads: According to the power-law model, the rate of fitness gain declines over time but fitness has no upper limit, whereas the hyperbolic model implies a hard limit. ... Consistent with the previous study, the power-law model fits the new data better than the hyperbolic model.
Qualifies
Sustained fitness gains and variability in fitness trajectories in the long-term evolution experiment with Escherichia coliabstract, last sentence
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: both adaptation and divergence can continue indefinitely–or at least for a long time–even in a constant environment
What this does not establish
A better fit between two models, not proof that no ceiling exists. The authors themselves write "or at least for a long time".
Claim 21 · checked 24 September 2026 · AI-assisted audit
Claim: Those nine did not include the one that did something unusual, which was left out of the measurements.
- Kind
- Historical claim. A statement about the past that historical evidence can test.
- Review
- Source checked. The cited passage was read, and it says this.
Supports
Sustained fitness gains and variability in fitness trajectories in the long-term evolution experiment with Escherichia coliMaterial and methods (a)
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: Our analyses used whole-population samples from nine of the 12 LTEE populations ... that no longer make colonies that can be reliably counted in the standard fitness assays or that evolved the ability to consume the citrate in the culture medium, which also precludes using the standard assays
Corrected
24 September 2026. Previously: “That is a result about all twelve lines rather than about the one that did something unusual, and it is the more general finding of the two.” The 2015 paper measured nine of the twelve populations and excluded three, including the one that evolved citrate use.
Claim 22 · checked 24 September 2026 · AI-assisted audit
Claim: It is a result about ordinary adaptation, and the more general finding of the two.
- Kind
- This page’s reading. A judgement the page makes from the evidence. Others read it differently.
- Review
- A judgement, not a fact. Reviewed for whether it is fairly put and follows from the checked claims. No source can confirm it.
The page’s judgement: the fitness result covers nine populations adapting to the same medium, the citrate result one population and one trait.
Claim 23 · checked 24 September 2026 · AI-assisted audit
And one population did something else
Claim: And one population did something else
- Kind
- Observation. Something measured or seen, as the named source reports it.
- Review
- Source checked. The cited passage was read, and it says this.
Supports
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: finally evolved in one population by 31,500 generations, causing an increase in population size and diversity
Later work looked for
Europe PMC, 2012 on, "long-term evolution experiment" and citrate: Leon and colleagues (2018) report citrate use still in only one of the twelve populations after 30 years and find more than one potentiating step; Van Hofwegen and colleagues (2016) dispute the rarity. Both added and the page updated.
Claim 24 · checked 24 September 2026 · AI-assisted audit
Claim: The growth medium contains citrate, which this bacterium is characteristically unable to use in the presence of oxygen.
- Kind
- Observation. Something measured or seen, as the named source reports it.
- Review
- Source checked. The cited passage was read, and it says this.
Supports
Historical contingency and the evolution of a key innovation in an experimental population of Escherichia coliintroduction, paragraph 6
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: DM25 medium contains not only glucose, but also citrate at a high concentration. The inability to use citrate as an energy source under oxic conditions has long been a defining characteristic of E. coli as a species
What this does not establish
A few strains isolated from farms and clinics use citrate, carrying genes acquired from other species on plasmids, and one earlier laboratory mutant was reported.
Later work looked for
Europe PMC, 2012 on, E. coli aerobic citrate: Van Hofwegen and colleagues (2016) begin from the same fact ("E. coli cannot use citrate aerobically"); nothing that changes it.
Claim 25 · checked 24 September 2026 · AI-assisted audit
Claim: After more than thirty thousand generations, one population began using it, and its population size jumped.
- Kind
- Observation. Something measured or seen, as the named source reports it.
- Review
- Source checked. The cited passage was read, and it says this.
The first variant used citrate weakly; the jump in population size came about two thousand generations later, after further mutations improved it.
Supports
Historical contingency and the evolution of a key innovation in an experimental population of Escherichia coliabstract; Discussion
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: finally evolved in one population by 31,500 generations, causing an increase in population size and diversity ... leading to a massive population expansion
Later work looked for
Europe PMC, 2012 on, "long-term evolution experiment" and citrate: Leon and colleagues (2018) report citrate use still in only one of the twelve populations after 30 years and find more than one potentiating step; Van Hofwegen and colleagues (2016) dispute the rarity. Both added and the page updated.
Claim 26 · checked 24 September 2026 · AI-assisted audit
Claim: The key result came from replaying the tape.
- Kind
- This page’s reading. A judgement the page makes from the evidence. Others read it differently.
- Review
- A judgement, not a fact. Reviewed for whether it is fairly put and follows from the checked claims. No source can confirm it.
The page’s judgement of which part of the paper matters most.
Claim 27 · checked 24 September 2026 · AI-assisted audit
Claim: Clones revived from the frozen samples re-evolved the ability only if they had been taken at generation 20,000 or later
- Kind
- Experimental result. What an experiment or trial found, under its own conditions. A result in animals, cells or a simulation is not a result in people.
- Review
- Source checked. The cited passage was read, and it says this.
Supports
Historical contingency and the evolution of a key innovation in an experimental population of Escherichia coliResults, third replay experiment; Table 1
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: one (the one that produced two mutants) was from generation 20,000. ... nor among any of the other 600 cultures that used clones isolated before generation 20,000.
Supports
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: E. coli isolates from all of these groups evolved Cit+ in the replay experiments, whereas no strains from earlier than 20,000 generations did
What this does not establish
Three replay experiments, seventeen independent mutants in all. Even from later clones the ability arose rarely.
Later work looked for
Europe PMC, 2012 on, "long-term evolution experiment" and citrate: Leon and colleagues (2018) report citrate use still in only one of the twelve populations after 30 years and find more than one potentiating step; Van Hofwegen and colleagues (2016) dispute the rarity. Both added and the page updated.
Corrected
24 September 2026. Previously: “Restarting from frozen samples showed that the innovation only arose from populations descended from a particular point in that lineage’s history: an earlier mutation, harmless on its own, had made the later one possible.” The replays started from single clones, not populations. The 2008 paper did not identify the potentiating change and says it did not know whether it was beneficial, neutral or deleterious, so "harmless on its own" was unsupported; later work (Leon and colleagues, 2018) finds more than one change and a first step whose benefit changed over time.
Claim 28 · checked 24 September 2026 · AI-assisted audit
Claim: something earlier in that lineage’s history had made the step far more likely.
- Kind
- Hypothesis. A proposed explanation, not an established one.
- Review
- Source checked. The cited passage was read, and it says this.
Supports
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: indicating that some potentiating mutation arose by 20,000 generations. This potentiating change increased the mutation rate to
Qualifies
Rapid evolution of citrate utilization by Escherichia coli by direct selection requires citT and dctAabstract, Importance
Cited · Link answered, 19 October 2026 · Abstract only, 24 September 2026 · Research in microbes · Experiment
The source reads: We conclude that the rarity of the LTEE mutant was an artifact of the experimental conditions and not a unique evolutionary event.
What this does not establish
The authors’ inference from the replays. The 2008 paper did not identify the change.
Other readings
Van Hofwegen, Hovde and Minnich argue that the rarity reflects the experiment’s conditions rather than the lineage’s history.
Claim 29 · checked 24 September 2026 · AI-assisted audit
Claim: Later work found more than one such change.
- Kind
- Experimental result. What an experiment or trial found, under its own conditions. A result in animals, cells or a simulation is not a result in people.
- Review
- Source checked. The cited passage was read, and it says this.
Supports
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: suggests the existence of at least two critical junctures at which the potential for evolving Cit+ increased
What this does not establish
Leon and colleagues infer this from where on the family tree the replayed clones sat; the individual mutations are candidates, not all confirmed.
Later work looked for
Europe PMC, 2012 on, "long-term evolution experiment" and citrate: Leon and colleagues (2018) report citrate use still in only one of the twelve populations after 30 years and find more than one potentiating step; Van Hofwegen and colleagues (2016) dispute the rarity. Both added and the page updated.
Claim 30 · checked 24 September 2026 · AI-assisted audit
Claim: The first step toward using citrate was slightly beneficial even in the ancestor, became harmful as other mutations accumulated, and was beneficial again by generation 29,000.
- Kind
- Experimental result. What an experiment or trial found, under its own conditions. A result in animals, cells or a simulation is not a result in people.
- Review
- Source checked. The cited passage was read, and it says this.
Supports
Innovation in an E. coli evolution experiment is contingent on maintaining adaptive potential until competition subsidesabstract; Discussion
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: We found that there was already a slight fitness benefit for this mutation in the original ancestor ... the lineage in which Cit+ eventually evolved became ‘anti-potentiated’ ... it was deleterious to evolve rudimentary Cit+ in this genetic background ... further mutations appeared in the focal LTEE lineage by 29,000 generations
What this does not establish
Measured by adding the first-step mutation to clones from different generations and competing them in the experiment’s medium. One lineage, one trait.
Later work looked for
Europe PMC, 2012 on, "long-term evolution experiment" and citrate: Leon and colleagues (2018) report citrate use still in only one of the twelve populations after 30 years and find more than one potentiating step; Van Hofwegen and colleagues (2016) dispute the rarity. Both added and the page updated.
Corrected
24 September 2026. Previously: “” Added: the page’s account of a single harmless potentiating mutation was superseded by Leon and colleagues (2018).
Claim 31 · checked 24 September 2026 · AI-assisted audit
Claim: Its authors called it an explicit demonstration of historical contingency.
- Kind
- What scholars argue. A report of what specialists argue, or where they disagree.
- Review
- Source checked. The cited passage was read, and it says this.
Supports
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: provides an explicit demonstration of the importance of historical contingency in evolution
Corrected
24 September 2026. Previously: “That is contingency demonstrated rather than argued.” The reading is disputed (Van Hofwegen and colleagues, 2016), so it is attributed to the authors who hold it, and the dispute is reported in the next paragraph.
Claim 32 · checked 24 September 2026 · AI-assisted audit
Claim: The same environment, the same organism, and an outcome that depended on what had already happened by generation 20,000.
- Kind
- Disputed. A contested point, where the page reports a side or the disagreement.
- Review
- A judgement, not a fact. Reviewed for whether it is fairly put and follows from the checked claims. No source can confirm it.
Supports
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: Thus, the evolution of this phenotype was contingent on the particular history of that population.
Cuts against
Rapid evolution of citrate utilization by Escherichia coli by direct selection requires citT and dctAabstract, Importance
Cited · Link answered, 19 October 2026 · Abstract only, 24 September 2026 · Research in microbes · Experiment
The source reads: We conclude that the rarity of the LTEE mutant was an artifact of the experimental conditions and not a unique evolutionary event.
Other readings
Van Hofwegen, Hovde and Minnich read the delay as a product of the experiment’s conditions rather than of the lineage’s history; the next paragraph reports both sides.
Corrected
24 September 2026. Previously: “and an outcome that depended on what had happened twenty thousand generations earlier.” The potentiating change arose by generation 20,000 and citrate use about 31,500, so it came about eleven thousand generations earlier, not twenty thousand.
Claim 33 · checked 24 September 2026 · AI-assisted audit
Claim: Not everyone reads it that way. Van Hofwegen, Hovde and Minnich selected directly for citrate use and got it within 12 to 100 generations, through the same class of mutation, and conclude that the rarity in the long experiment was an artefact of its conditions.
- Kind
- What scholars argue. A report of what specialists argue, or where they disagree.
- Review
- Partly checked. Only part of the source could be read. What was not read is listed.
Supports
Cited · Link answered, 19 October 2026 · Abstract only, 24 September 2026 · Research in microbes · Experiment
The source reads: Potentiation/actualization mutations occurred within as few as 12 generations, and refinement mutations occurred within 100 generations. ... These mutations were equivalent to the amplification and promoter capture CitT-activating mutations identified in the LTEE. ... We conclude that the rarity of the LTEE mutant was an artifact of the experimental conditions
What this does not establish
Only the abstract was read. Their selection used citrate-rich media in E. coli B and K-12, not the long experiment’s daily transfers in glucose.
Later work looked for
Europe PMC, 2012 on, "long-term evolution experiment" and citrate: Leon and colleagues (2018) report citrate use still in only one of the twelve populations after 30 years and find more than one potentiating step; Van Hofwegen and colleagues (2016) dispute the rarity. Both added and the page updated.
Corrected
24 September 2026. Previously: “” Added: the page presented contingency in this experiment as settled; a published rival reading exists and the authors’ side has answered it.
Claim 34 · checked 24 September 2026 · AI-assisted audit
Claim: Leon and colleagues answer that starving cells on citrate masks the dependence on genetic background that the long experiment exposes.
- Kind
- What scholars argue. A report of what specialists argue, or where they disagree.
- Review
- Source checked. The cited passage was read, and it says this.
The paragraph cites Van Hofwegen and colleagues among the studies it answers.
Supports
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: these stark conditions are expected to completely mask and overwhelm the dependency on potentiating genetic and population factors found in the LTEE
Claim 35 · checked 24 September 2026 · AI-assisted audit
Claim: What the replays showed
- Kind
- Framing. Signposting. It asserts nothing a source could check.
Claim: Later generations of one lineage produced the innovation repeatedly.
- Kind
- Experimental result. What an experiment or trial found, under its own conditions. A result in animals, cells or a simulation is not a result in people.
- Review
- Source checked. The cited passage was read, and it says this.
Supports
Historical contingency and the evolution of a key innovation in an experimental population of Escherichia coliResults, statistical analysis of the replay experiments
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: All three experiments show the same tendency for ... variants to evolve more often from clones sampled in later than earlier generations of population Ara-3
What this does not establish
Repeatedly, but rarely: seventeen independent mutants across three experiments.
Later work looked for
Europe PMC, 2012 on, "long-term evolution experiment" and citrate: Leon and colleagues (2018) report citrate use still in only one of the twelve populations after 30 years and find more than one potentiating step; Van Hofwegen and colleagues (2016) dispute the rarity. Both added and the page updated.
Claim 37 · checked 24 September 2026 · AI-assisted audit
Claim: Something in that lineage’s background made it far more likely.
- Kind
- Hypothesis. A proposed explanation, not an established one.
- Review
- Source checked. The cited passage was read, and it says this.
Supports
Historical contingency and the evolution of a key innovation in an experimental population of Escherichia coliabstract; Results, mutation-rate experiment
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: indicating that some potentiating mutation arose by 20,000 generations ... The potentiated genetic background thus increases the mutation rate to ... 2-fold, and probably much more
Other readings
Van Hofwegen, Hovde and Minnich attribute the rarity to the experiment’s conditions rather than to the lineage.
Corrected
24 September 2026. Previously: “Something in that lineage’s background made it reachable.” Leon and colleagues (2018) find the first step was possible, and slightly beneficial, even in the ancestor; what changed was how likely it was to appear and succeed.
Claim 38 · checked 24 September 2026 · AI-assisted audit
Claim: Earlier generations of the same lineage never did, in many replays.
- Kind
- Experimental result. What an experiment or trial found, under its own conditions. A result in animals, cells or a simulation is not a result in people.
- Review
- Source checked. The cited passage was read, and it says this.
Supports
Historical contingency and the evolution of a key innovation in an experimental population of Escherichia coliResults, third replay experiment
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: nor among any of the other 600 cultures that used clones isolated before generation 20,000.
Later work looked for
Europe PMC, 2012 on, "long-term evolution experiment" and citrate: Leon and colleagues (2018) report citrate use still in only one of the twelve populations after 30 years and find more than one potentiating step; Van Hofwegen and colleagues (2016) dispute the rarity. Both added and the page updated.
Claim 39 · checked 24 September 2026 · AI-assisted audit
Claim: Whatever it was had arisen by generation 20,000.
- Kind
- Hypothesis. A proposed explanation, not an established one.
- Review
- Source checked. The cited passage was read, and it says this.
Supports
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: indicating that some potentiating mutation arose by 20,000 generations
Qualifies
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: further mutations appeared in the focal LTEE lineage by 29,000 generations that altered the fitness impact of activating citT expression such that it was again beneficial
What this does not establish
The first such change; later work finds another by generation 29,000.
Corrected
24 September 2026. Previously: “A potentiating mutation had to come first. The step was not available before it.” Leon and colleagues (2018) find the first step was available, and slightly beneficial, in the ancestor, and more than one change was involved; the 2008 paper supports only that potentiation had occurred by generation 20,000.
Claim 40 · checked 24 September 2026 · AI-assisted audit
Claim: The other eleven populations never did, in tens of thousands of generations.
- Kind
- Observation. Something measured or seen, as the named source reports it.
- Review
- Source checked. The cited passage was read, and it says this.
Supports
Historical contingency and the evolution of a key innovation in an experimental population of Escherichia coliResults; Discussion
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: We therefore screened the other 11 populations, in most cases by using samples from generation 41,500. ... function, whereas all of the others remain
Supports
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: it still has evolved in only one of the twelve E. coli populations after 30 years of the Lenski experiment
Later work looked for
Europe PMC, 2012 on, "long-term evolution experiment" and citrate: Leon and colleagues (2018) report citrate use still in only one of the twelve populations after 30 years and find more than one potentiating step; Van Hofwegen and colleagues (2016) dispute the rarity. Both added and the page updated.
Claim 41 · checked 24 September 2026 · AI-assisted audit
Claim: History shaped what came next, in an experiment designed to remove every other difference.
- Kind
- Disputed. A contested point, where the page reports a side or the disagreement.
- Review
- A judgement, not a fact. Reviewed for whether it is fairly put and follows from the checked claims. No source can confirm it.
Supports
Historical contingency and the evolution of a key innovation in an experimental population of Escherichia coliintroduction, paragraph 3
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: Environmental conditions have been controlled, constant, and identical for all 12 lines.
Cuts against
Rapid evolution of citrate utilization by Escherichia coli by direct selection requires citT and dctAabstract, Importance
Cited · Link answered, 19 October 2026 · Abstract only, 24 September 2026 · Research in microbes · Experiment
The source reads: We conclude that the rarity of the LTEE mutant was an artifact of the experimental conditions and not a unique evolutionary event.
Other readings
Van Hofwegen, Hovde and Minnich read the outcome as shaped by the experiment’s conditions rather than by the lineage’s history.
Corrected
24 September 2026. Previously: “History constrains what is possible next, in an experiment designed to remove every other difference.” Leon and colleagues (2018) find the first step was possible from the start; history changed how likely it was, not whether it was possible.
Claim 42 · checked 24 September 2026 · AI-assisted audit
What it generalises to
Claim: What it generalises to
- Kind
- Framing. Signposting. It asserts nothing a source could check.
Claim: A frozen sample every five hundred generations is a fossil record you can restart.
- Kind
- Observation. Something measured or seen, as the named source reports it.
- Review
- Source checked. The cited passage was read, and it says this.
Supports
Historical contingency and the evolution of a key innovation in an experimental population of Escherichia coliintroduction, paragraph 3
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Experiment
The source reads: samples have been frozen every 500 generations, providing a rich ... fossil record ... these samples remain viable
Later work looked for
Europe PMC, 2012 on, "long-term evolution experiment": archived samples are still revived in later work (Leon and colleagues, 2018); nothing that changes it.
Claim 44 · checked 24 September 2026 · AI-assisted audit
How much is contingency
Claim: How much is contingency
- Kind
- Framing. Signposting. It asserts nothing a source could check.
Claim: Convergence is common, perhaps ubiquitous: eyes, wings, echolocation and streamlined bodies have appeared repeatedly in unrelated lineages
- Kind
- What the field holds. The settled view of a field, as a review, a consensus statement or a reference work gives it. Not one study.
- Review
- Source checked. The cited passage was read, and it says this.
Supports
Convergence, adaptation, and constraintintroduction; section on genotypes and phenotypes
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Texts · Review of the field
The source reads: is a common, perhaps ubiquitous ... ichthyosaurs and dolphins to evolve a streamlined body form ... wings of birds and bats ... Cetaceans and bats have independently acquired 14 identical amino acid changes in Prestin ... adaptations involved in the evolution of echolocating capability in the two groups ... eyes of mollusks, vertebrates, and invertebrates
What this does not establish
"Perhaps ubiquitous" is Conway Morris’s position as Losos reports it; the review does not measure how common convergence is.
Later work looked for
Europe PMC, 2011 on, convergent evolution review: nothing that changes the description of convergence as common.
Corrected
24 September 2026. Previously: “Convergence is everywhere: eyes, wings, echolocation and body plans” The cited review says "common, perhaps ubiquitous", not everywhere, and gives streamlined body form as its example rather than body plans.
Claim 46 · checked 24 September 2026 · AI-assisted audit
Claim: which argues that selection finds the same solutions given the same problems.
- Kind
- What scholars argue. A report of what specialists argue, or where they disagree.
- Review
- Source checked. The cited passage was read, and it says this.
The argument is chiefly Simon Conway Morris’s, against Gould.
Supports
Historical contingency and the evolution of a key innovation in an experimental population of Escherichia coliintroduction, paragraph 2
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Texts · Review of the field
The source reads: He and others point to numerous examples of convergent evolution as evidence that selection finds the same adaptations despite the vagaries of history.
Supports
Convergence, adaptation, and constraintintroduction
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Texts · Review of the field
The source reads: Many see in convergent evolution the unfettered ability of natural selection to produce optimal solutions to problems repeatedly posed by the environment.
Claim 47 · checked 24 September 2026 · AI-assisted audit
Claim: The argument survives examination and does not survive it unqualified.
- Kind
- This page’s reading. A judgement the page makes from the evidence. Others read it differently.
- Review
- A judgement, not a fact. Reviewed for whether it is fairly put and follows from the checked claims. No source can confirm it.
The page’s summary of the review reported in the next sentence.
Claim 48 · checked 24 September 2026 · AI-assisted audit
Claim: Convergence in similar environments often is selection at work, it can also happen for other reasons, and species under the same pressure sometimes answer it with adaptations that are not convergent at all.
- Kind
- What scholars argue. A report of what specialists argue, or where they disagree.
- Review
- Source checked. The cited passage was read, and it says this.
Supports
Convergence, adaptation, and constraintabstract
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Texts · Review of the field
The source reads: I reaffirm that convergence in taxa occupying similar selective environments often is the result of natural selection. However, convergent evolution of a trait in a particular environment can occur for reasons other than selection on that trait in that environment
Supports
Convergence, adaptation, and constraintabstract
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Texts · Review of the field
The source reads: species can respond to similar selective pressures by evolving non-convergent adaptations
Claim 49 · checked 24 September 2026 · AI-assisted audit
Claim: Both observations are correct, which means the question is quantitative rather than binary.
- Kind
- This page’s reading. A judgement the page makes from the evidence. Others read it differently.
- Review
- A judgement, not a fact. Reviewed for whether it is fairly put and follows from the checked claims. No source can confirm it.
Follows from the parallel changes and the citrate result reported above, and from the review’s account of convergence.
Context
Convergence, adaptation, and constraintsection on genotypes and phenotypes, closing
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Texts · Review of the field
The source reads: parsing the relative roles of natural selection and constraint in determining the extent and form of biological diversity can be context-dependent and at times subjective
Claim 50 · checked 24 September 2026 · AI-assisted audit
Claim: How much of what exists is inevitable and how much is an accident nobody can currently say, because the experiment that would tell us is the history of the planet.
- Kind
- Open question. Something the page says is not known.
- Review
- Presented as open. The page says this is unresolved, and the review found that it is.
Supports
Historical contingency and the evolution of a key innovation in an experimental population of Escherichia coliintroduction, paragraph 3
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Texts · Review of the field
The source reads: replaying life’s tape on the planetary scale is impossible
Claim 51 · checked 24 September 2026 · AI-assisted audit
Whether prediction is useful in practice
Claim: Whether prediction is useful in practice
- Kind
- Framing. Signposting. It asserts nothing a source could check.
Claim: Short-term evolution is forecast in practice.
- Kind
- Observation. Something measured or seen, as the named source reports it.
- Review
- Source checked. The cited passage was read, and it says this.
Supports
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Model
The source reads: Experts select strains based on experimental measurements of antigenic drift and predictions made by models from hemagglutinin sequences.
Supports
Integrating genotypes and phenotypes improves long-term forecasts of seasonal influenza A/H3N2 evolutionDiscussion, first paragraph
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Model
The source reads: We have added this framework as a component of seasonal influenza analyses on nextstrain.org where it provides real-time forecasts for influenza researchers, decision makers, and the public.
What this does not establish
The example is seasonal influenza; the page does not claim this for other organisms.
Later work looked for
Europe PMC, 2015 on, influenza forecasting and fitness models: Huddleston and colleagues (2020) describe current use and improved forecasts; added and the page updated.
Corrected
24 September 2026. Previously: “Short-term evolution is predicted operationally.” The Łuksza and Lässig paper proposes a method; that such forecasts are used in vaccine strain selection is carried by Huddleston and colleagues (2020), now cited.
Claim 53 · checked 24 September 2026 · AI-assisted audit
Claim: A fitness model for the influenza surface protein, inferred from the sequences of strains already circulating, predicts how common each strain’s descendants will be the following year,
- Kind
- What a model says. What a theory or model predicts or describes, which is not the same as what has been observed.
- Review
- Partly checked. Only part of the source could be read. What was not read is listed.
Supports
A predictive fitness model for influenzaabstract
Cited · Link answered, 19 October 2026 · Abstract only, 24 September 2026 · Research in microbes · Model
The source reads: We infer both fitness components for the strains circulating in a given year, using population-genetic data of all previous strains. From fitness and frequency of each strain, we predict the frequency of its descendent strains in the following year.
What this does not establish
Only the abstract was read, so how accurate the predictions were is not reported here. One subtype, A/H3N2, one year ahead.
Corrected
24 September 2026. Previously: “predicts which of them will dominate the following year” The model predicts the frequency of each strain’s descendants, which is what the abstract says, not which strain will dominate.
Claim 54 · checked 24 September 2026 · AI-assisted audit
Claim: and was published as a principled basis for choosing what goes into the vaccine.
- Kind
- Historical claim. A statement about the past that historical evidence can test.
- Review
- Partly checked. Only part of the source could be read. What was not read is listed.
Supports
A predictive fitness model for influenzaabstract
Cited · Link answered, 19 October 2026 · Abstract only, 24 September 2026 · Research in microbes · Model
The source reads: This fitness model maps the adaptive history of influenza A and suggests a principled method for vaccine selection.
What this does not establish
Only the abstract was read.
Claim 55 · checked 24 September 2026 · AI-assisted audit
Claim: Experts now choose vaccine strains using forecasts of this kind alongside laboratory measurements,
- Kind
- Observation. Something measured or seen, as the named source reports it.
- Review
- Source checked. The cited passage was read, and it says this.
Supports
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Model
The source reads: Experts select strains based on experimental measurements of antigenic drift and predictions made by models from hemagglutinin sequences.
Supports
Integrating genotypes and phenotypes improves long-term forecasts of seasonal influenza A/H3N2 evolutionIntroduction, paragraph 5
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Model
The source reads: unpublished work by Luksza and Lässig, 2014 to the WHO GISRS network incorporates antigenic phenotypes into fitness-based predictions
What this does not establish
The paper describes strain selection by the WHO network for influenza A/H3N2; how much weight the forecasts carry in each decision is not reported.
Later work looked for
Europe PMC, 2015 on, influenza forecasting: Huddleston and colleagues (2020); added.
Corrected
24 September 2026. Previously: “” Added from newer work: the page described a 2014 method without saying how it is used or whether it has been bettered.
Claim 56 · checked 24 September 2026 · AI-assisted audit
Claim: and forecasts that add those measurements of how the virus escapes antibodies do better than sequences alone.
- Kind
- What a model says. What a theory or model predicts or describes, which is not the same as what has been observed.
- Review
- Source checked. The cited passage was read, and it says this.
Supports
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Model
The source reads: Forecasts informed by phenotypic measures of antigenic drift consistently outperformed previous sequence-only estimates
Qualifies
Integrating genotypes and phenotypes improves long-term forecasts of seasonal influenza A/H3N2 evolutionDiscussion, Forecasting framework aids practical forecasts
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Model
The source reads: the average gain from these models over the naive model appears low at 0.96 AAs per year for validation data and 0.85 AAs per year for test data
What this does not establish
Measured by forecasting past seasons of one subtype, A/H3N2. The authors call the gain over a model that predicts no change low, under one amino acid per year.
Claim 57 · checked 24 September 2026 · AI-assisted audit
Claim: Those predictions are statistical and they are about the next step.
- Kind
- This page’s reading. A judgement the page makes from the evidence. Others read it differently.
- Review
- A judgement, not a fact. Reviewed for whether it is fairly put and follows from the checked claims. No source can confirm it.
Follows from the one-year horizon both papers describe.
Context
Cited · Link answered, 19 October 2026 · Read in full, 24 September 2026 · Research in microbes · Model
The source reads: Effective vaccine strains need to represent H3N2 populations circulating one year after strain selection.
Claim 58 · checked 24 September 2026 · AI-assisted audit
Claim: They do not extend to what a lineage will look like in a million years, and nothing suggests they could.
- Kind
- This page’s reading. A judgement the page makes from the evidence. Others read it differently.
- Review
- A judgement, not a fact. Reviewed for whether it is fairly put and follows from the checked claims. No source can confirm it.
The page’s judgement. Neither forecasting paper claims more than a year ahead.
Claim 59 · checked 24 September 2026 · AI-assisted audit
What was read
- Historical contingency and the evolution of a key innovation in an experimental population of Escherichia coliBlount, Borland and Lenski, Proceedings of the National Academy of Sciences · Publisher PDF, PNAS 105(23):7899-7906, archived at web.archive.org/web/2020/https://www.pnas.org/content/pnas/105/23/7899.full.pdfRead in full, 24 September 2026 · Copyright; quoted brieflyThe supporting information was not read. Europe PMC and PMC do not serve this article as XML.
- Convergence, adaptation, and constraintLosos, Evolution 65 · Author manuscript, Harvard DASH (urn-3:HUL.InstRepos:9938750), archived by the Wayback MachineRead in full, 24 September 2026 · Harvard Open Access Policy; quoted brieflyThe manuscript, not the typeset version in Evolution 65(7):1827-1840.
- A predictive fitness model for influenzaŁuksza and Lässig, Nature 507 · Europe PMC record, PMID 24572367Abstract only, 24 September 2026 · Copyright; quoted brieflyThe paper is closed; no open copy was found through Europe PMC, unpaywall or Semantic Scholar.
- Sustained fitness gains and variability in fitness trajectories in the long-term evolution experiment with Escherichia coliLenski and others, Proceedings of the Royal Society B · Europe PMC full text, PMC4707762Read in full, 24 September 2026 · Open access; quoted brieflyThe electronic supplementary tables were not read.
- Innovation in an E. coli evolution experiment is contingent on maintaining adaptive potential until competition subsidesLeon, D'Alton, Quandt and Barrick, PLOS Genetics 14 · Europe PMC full text, PMC5918244Read in full, 24 September 2026 · CC BY 4.0
- Rapid evolution of citrate utilization by Escherichia coli by direct selection requires citT and dctAVan Hofwegen, Hovde and Minnich, Journal of Bacteriology 198 · Europe PMC record; NCBI PMC4800869 serves the abstract onlyAbstract only, 24 September 2026 · Copyright; quoted brieflyThe publisher does not allow the full text in XML; the body was not read.
- Integrating genotypes and phenotypes improves long-term forecasts of seasonal influenza A/H3N2 evolutionHuddleston and others, eLife 9 · Europe PMC full text, PMC7553778 (eLife 9:e60067)Read in full, 24 September 2026 · CC0 1.0 public domain dedicationRead in full in the integrity pass; first recorded from the abstract.